Search results for "methods [Neuroimaging]"

FICC-Seq: a method for enzyme-specified profiling of methyl-5-uridine in cellular RNA.

2019

AbstractMethyl-5-uridine (m5U) is one the most abundant non-canonical bases present in cellular RNA, and in yeast is found at position U54 of tRNAs where modification is catalysed by the methyltransferase Trm2. Although the mammalian enzymes that catalyse m5U formation are yet to be identified via experimental evidence, based on sequence homology to Trm2, two candidates currently exist, TRMT2A and TRMT2B. Here we developed a genome-wide single-nucleotide resolution mapping method, Fluorouracil-Induced-Catalytic-Crosslinking-Sequencing (FICC-Seq), in order to identify the relevant enzymatic targets. We demonstrate that TRMT2A is responsible for the majority of m5U present in human RNA, and t…

Approaches to study yeast cell aging and death

2014

For millennia, yeast has been exploited to obtain fermentation products, such as foods and beverages. For c. 50 years, yeast has been an established model organism for basic and applied research, and more specifically, for c. 15 years, this unicellular organism has been applied to dissect molecular mechanisms of cell aging and programmed cell death. In this review, we present an overview of approaches to study cell aging and death in yeast, including lifespan assessments, calorie restriction, cell viability, survival, and death markers.

Analysis and modeling of Temporal Dominance of Sensations with stochastic processes

2019

Temporal Dominance of Sensations (TDS) is a technique to measure temporal perception of food product during tasting. For a panelist, it consists in choosing in a list of attributes which one is dominant at any time. This work aims to model TDS data with a stochastic process and proposes to use semi-Markov processes (SMP), a generalization of Markov chains which allows dominance durations to be modeled by any type of distribution. The model can then be used to compare TDS samples based on likelihood ratio. Because probabilities of transition from one attribute to another one can also depend on time, we propose to model TDS by period and we propose a method to select optimally the number of p…

Morphometry of Middle Bronze Age palstaves by discrete cosine transform.

2009

9 pages; International audience; The Discrete Cosine Transform (DCT) is a Fourier-related transform widely used in signal processing and well suited to the analysis of open outlines. This method was applied here to evaluate the discrimination power of the inner lateral rib for two palstave populations dating from the Middle Bronze Age, excavated in northwest France. A corpus of almost 400 palstaves (bronze axes) of the Breton and Norman types was processed, and compared to specimens found at Sermizelles in Burgundy. The procedure is robust and produces a discrimination in good agreement with the traditional typology. Besides the definition of a ‘standard' shape for each population, the morp…

Inland harbours in Central Europe:: Junctions between Northern Europe and the Mediterranean Sea

2016

The international symposium organized in Dijon offers a structural and diachronic comparative analysis of the harbours networks on inland waterbodies. Its goals are the study of the harbours settled on lakes or rivers as a complex phenomenon, and the assessment of their respective significance as hubs in the communication and exchange network between Mediterranean Sea and Northern Europe.These two days gather German, French and European researchers from the different fields required on such a research problem (history, archaeology,geography, palaeoenvironmental analysis, geomatics), in the aim to present recent discoveries and results.The present book contains the 27 abstracts in three lang…

2015

We examined the effects of spatial frequency similarity and dissimilarity on human contour integration under various conditions of uncertainty. Participants performed a temporal 2AFC contour detection task. Spatial frequency jitter up to 3.0 octaves was applied either to background elements, or to contour and background elements, or to none of both. Results converge on four major findings. (1) Contours defined by spatial frequency similarity alone are only scarcely visible, suggesting the absence of specialized cortical routines for shape detection based on spatial frequency similarity. (2) When orientation collinearity and spatial frequency similarity are combined along a contour, performa…

Study of published articles on management accounting in Brazil and Spain

2013

En las últimas décadas, se han producido cambios importantes en la contabilidad de gestión con la inclusión de nuevos temas y métodos de investigación, revistas exclusivas y, en especial, estudios con perspectivas multidisciplinarias. Estos cambios se han detectado con estudios divulgados en publicaciones de revistas importantes. En este contexto surge la siguiente pregunta de investigación: ¿Cuál es el perfil de las investigaciones a respecto de la contabilidad de gestión en España y Brasil? Así, el objetivo del presente trabajo es identificar y analizar los temas y métodos de investigación aplicados en los estudios de contabilidad de gestión en España y Brasil, presentar cómo esas investi…

Gain-scheduled H-infinity observer design for nonlinear stochastic systems with time-delay and actuator saturation

2012

In this paper, we propose a method for designing continuous gain-scheduled robust H ∞ observer on a class of extended stochastic nonlinear systems subject to time delay and actuator saturation. Initially, gradient linearization procedure is applied to describe such extended nonlinear systems into several model-based linear systems. Next, a robust linear H ∞ observer is designed to such linear stochastic models. Subsequently, a convex hull set is investigated and sufficient condition is derived in terms of feedback observer to determine whether a given initial condition belongs to an ellipsoid invariant set. Finally, continuous gain-scheduled approach is employed to design continuous nonline…

Distribution Amplitudes of Heavy-Light Mesons

2019

A symmetry-preserving approach to the continuum bound-state problem in quantum field theory is used to calculate the masses, leptonic decay constants and light-front distribution amplitudes of empirically accessible heavy-light mesons. The inverse moment of the $B$-meson distribution is particularly important in treatments of exclusive $B$-decays using effective field theory and the factorisation formalism; and its value is therefore computed: $\lambda_B(\zeta = 2\,{\rm GeV}) = 0.54(3)\,$GeV. As an example and in anticipation of precision measurements at new-generation $B$-factories, the branching fraction for the rare $B\to \gamma(E_\gamma) \ell \nu_\ell$ radiative decay is also calculated…

Illumina-based RiboMethSeq approach for mapping of 2'-O-Me residues in RNA

2016

International audience; RNA 2'-O-methylation is one of the ubiquitous nucleotide modifications found in many RNA types from Bacteria, Archaea and Eukarya. RNAs bearing 2'-O-methylations show increased resistance to degradation and enhanced stability in helices. While the exact role of each 2'-O-Me residue remained elusive, the catalytic protein Fibrillarin (Nop1 in yeast) responsible for 2'-O-methylation in eukaryotes, is associated with human pathologies. Therefore, there is an urgent need to precisely map and quantify hundreds of 2'-O-Me residues in RNA using high-throughput technologies. Here, we develop a reliable protocol using alkaline fragmentation of total RNA coupled to a commonly …